久久久天堂国产精品女人,无码午夜福利片,天天摸夜夜摸夜夜狠狠摸,欧美亚洲精品一区二三区

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>HUVECHUV-EC細(xì)胞, 人臍靜脈內(nèi)皮細(xì)胞

HUV-EC細(xì)胞, 人臍靜脈內(nèi)皮細(xì)胞

簡要描述:HUV-EC-C細(xì)胞, 人臍靜脈內(nèi)皮細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號:HUVEC
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-16
  • 訪  問  量:2178

產(chǎn)品分類

Product Category

詳細(xì)介紹

HUV-EC-C細(xì)胞, 人臍靜脈內(nèi)皮細(xì)胞

ATCC® Number:CRL-1730™    Price:$280.00
DeBiosSesignations:HUV-EC-C

rum:afety Level:1

Shipped:frozen

Medium &See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:endothelial

Source:Organ: umbilical vein
Tissue: vascular endothelium
Disease: normal
Cell Type: endothelial


Cellular Products:factor VIII [23284]

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
HUV-EC-C細(xì)胞, 人臍靜脈內(nèi)皮細(xì)胞

Applications:transfection host (technology from amaxa)

Tumorigenic:No

DNA Profile (STR):Amelogenin: X

CSF1PO: 11,12

D13S317: 9,11

D16S539: 11,12

D5S818: 11,12

D7S820: 8,12

THO1: 6,9.3

TPOX: 8,11

vWA: 16



Cytogenetic Analysis:This is a hypodiploid human cell line. The modal chromosome number was 45 occurring in 72% of cells counted. The rate of polyploid cells was 15.8%. All cells had monosomic N13 and the subclone with additional monosomic N15 predominates. Other coexisting subclones include those with 46,XX,-11,-13,i(11p),i(11q) and 46,XX,+11,-13 karyotypes. Both X chromosomes appear normal.

Comments:Endothelial Cell Growth Supplement (ECGS) and unidentified factors from bovine pituitary, hypothalamus or whole brain extracts are mitogenic for this line. [23284]

The cells have a life expectancy of 50 to 60 population doublings.



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated of F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: 0.1 mg/ml heparin; 0.03-0.05 mg/ml endothelial cell growth supplement (ECGS); adjust to a final concentration of 10% fetal bovine serum.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended
Medium Renewal: Two to three times per week


Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2020



References:23284: Hoshi H, McKeehan WL. Brain- and liver cell-derived factors are required for growth of human endothelial cells in serum-free culture. Proc. Natl. Acad. Sci. USA 81: 6413-6417, 1984. PubMed: 6333682
29192: Zahedi K. Characterization of the binding of serum amyloid P to laminin. J. Biol. Chem. 272: 2143-2148, 1997. PubMed: 8999915
33021: Soker S, et al. Characterization of novel vascular endothelial growth factor (VEGF) receptors on tumor cells that bind VEGF165 via its exon 7-endoded domain. J. Biol. Chem. 271: 5761-5767, 1996. PubMed:8621443























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
美妇| 日韩免费无码一区二区视频| 国产玖玖| 性按摩xxxx在按摩室| 有色网站| 最新超碰| 一本之道高清码11| 欧美丰满xxxaaa片| 久久一级| 乐色| 国产精品久久毛片| 亚洲av吞精久久久久| 精品国产乱码久久久久久口爆| 欧洲区乱码一二三| 国产中文区二暮区2021| 在线看日韩| 人妻被按摩师玩弄到潮喷| 亚洲高清偷拍一区二区三区| 亚洲中文字幕AV无码专区| 在线 | 一区二区三区| 岛国无码AV不卡一区二区| 中文字幕大看蕉在线观看| 国产av高清无亚洲| 久久亚洲中文字幕精品一区| 亚洲综合日韩久久成人AV| 国产在线精品一区二区高清不卡| 天等县| 万安县| 成人无码AV网站在线观看| 国产精品无码日韩欧| 成人国产亚洲精品a区| 亚洲欧洲中文日韩AV乱码 | 三级短视频| 欧美社区| 日本家庭影院| 沁源县| 亚洲精品欧美二区三区中文字幕| 在线观看无码的免费网站| 久久A级毛片免费观看| 熟妇高潮一区二区三区| 又爽又黄又无遮挡的视频1000|