久久久天堂国产精品女人,无码午夜福利片,天天摸夜夜摸夜夜狠狠摸,欧美亚洲精品一区二三区

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>HTB-1J82 細(xì)胞,人膀胱移行細(xì)胞癌細(xì)胞

J82 細(xì)胞,人膀胱移行細(xì)胞癌細(xì)胞

簡(jiǎn)要描述:J82 細(xì)胞,人膀胱移行細(xì)胞癌細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):HTB-1
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問(wèn)  量:1617

產(chǎn)品分類

Product Category

詳細(xì)介紹

J82 細(xì)胞,人膀胱移行細(xì)胞癌細(xì)胞

ATCC® Number:HTB-1™ Price:
Designations:J82Biosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiensMorphology:epithelial Source:Organ: urinary bladder Disease: transitional cell carcinomaPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining thAntigen Expression:HLA A2, Aw32, B5, B12, Cw5; Bloode permits. Please click here for information regarding the specific requirements for shipment to your location.Tumorigenic:YesType ADNA Profile (STR):Amelogenin: X,Y CSF1PO: 10,11 D13S317: 10,12 D16S539: 11,12 D5S818: 12,13 D7S820: 9,11 THO1: 9.3 TPOX: 11,12 vWA: 17,18Cytogenetic Analysis:The cell line is aneuploid human male (XY), with most chromosome counts in the triploid range. However, the chromosome count range is quite broad, extending from hyperdiploid to hexaploid. Normal chromosomes N11 and N20 are under-represented with respect to the other normal chromosomes: altered forms of these two chromosomes are prominent as marker chromosomes. Chromosome N13 tends towards over-representation. Five marker chromosomes are noted: 20q+, 11q+, 8p+, del(1)(q31), 5p+(HSR). One of these, 20q, is identical to marker chromosome M1 of C. O'Toole, et al., Br. J. Cancer 38: 64, 1978. The remainder are not as clearly related to the original marker chromosomes noted by those authors.Isoenzymes:AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1-2 PGM1, 1 PGM3, 2Age:58 yearsGender:male                       J82 細(xì)胞,人膀胱移行細(xì)胞癌細(xì)胞Ethnicity:Caucasian, SwedishComments:Electron microscopic examination did not reveal desmosomes but varying amounts of rough endoplasmic reticulum and prominent microfilaments were observed. Contains the ras (H-ras) oncogene.Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°CSubculturing:Protocol:Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:10 is recommended Medium Renewal: 2 to 3 times per weekPreservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phaseRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003recommended serum:ATCC 30-2020References:21849: O'Toole CHuman bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitroIn: O'Toole CIn vitro models for cancer researchvol. IVBoca Raton, FLCRC Presspp. 103-125.22325: O'Toole C, et al. Ultrastructure, karyology and immunology of a cell line originated from a human transitional-cell carcinoma. Br. J. Cancer 38: 64-76, 1978. PubMed: 68751924381: Fogh J. C*tion, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 57104725065: Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 901248432266: Bender CM, et al. Inhibition of DNA methylation by 5-Aza-2'-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res. 58: 95-101, 1998. PubMed: 9426064




















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
亚洲欧美精品一区| 老河口市| 天天射天天舔| 亚洲涩涩涩| 亚洲免费久久91无码国产| 伊人久久大香线蕉无码| 天天爽夜夜爽人人爽QC| 国产精品久久久久久久美男| 色哟哟入口国产精品| 99国产精品视频69v精品| 性暴力欧美猛交在线播放| 一区二区三区av波多野结衣| 欧美18videosex性欧美| 欧美啪啪网站| 一二三四| bbwbbw| 狠狠躁天天躁中文字幕| 韩国三级中文字幕HD| 青青久在线视频免费观看| 手机看片福利一区二区三区| 亚洲成AV人影院| 成人黄色免费网站| 人妻一二三区| 寂寞熟妇国产激情久久久| 久久99国产精品久久99小说| 无码av高潮喷水无码专区线| 国产精品一区二区香蕉| 亚洲精品区午夜亚洲精品区| 狠狠爱综合五月天婷婷| 美女露出奶头扒开尿口| 国产精品午夜无码体验区| 华宁县| 被男朋友口下面好爽| 亚洲一区日韩| 国产午夜一区二区三区| 人妻一二三区| 熟妇一区| 国内少妇情人精品av| 91女人18毛片水多国产| 亚洲第二页| 精品国产乱码一区二区三区|