久久久天堂国产精品女人,无码午夜福利片,天天摸夜夜摸夜夜狠狠摸,欧美亚洲精品一区二三区

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>NCI-H226人肺癌細(xì)胞株

人肺癌細(xì)胞株

簡要描述:CRL-5826 NCI-H226 人肺癌細(xì)胞株 ,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號:NCI-H226
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-12
  • 訪  問  量:2062

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-5826 NCI

-H226 人肺癌細(xì)胞株ATCC® Number: CRL-5826™

Designations: NCI-H226 [H226]

Depositors: AF Gazdar, JD Minna

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: Epithelial

CRL-5826 NCI-H226 人肺癌細(xì)胞株

Source: Organ: lung

Disease: squamous cell carcinoma; mesothelioma [23570]

Derived from metastatic site: pleural effusion

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. ephone (214) 699-8056, (214) 688-7233.

Isolation: Isolation date: March, 1980

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10,11

D13S317: 13,14

D16S539: 9,12

D5S818: 11,12

D7S820: 8,10

THO1: 8,9.3

TPOX: 8,11

vWA: 17

Cytogenetic Analysis: modal number = 47; range = 32-88; del(p25)

Gender: male

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37C.

Subc*tion Ratio: 1:4 to 1:8 is recommended

Medium Renewal: Every 2 to3 days

Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

References: 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996..


CRL-5826 NCI-H226


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
久久免费观看午夜成人网站| 日日躁夜夜躁狠狠躁超碰97| 色诱久久av| 欧美中日韩免费观看网站| 人妻 色综合网站| 99久久综合精品五月天| 浙江省| 少妇| 精品久久久久一区二区国产| 精品少妇人妻av一区二区三区| 欧美3区| 亚洲va欧美va天堂v国产综合| 99久久亚洲| 色综合欧美| 最新国产拍偷乱偷精品 | 亚洲大尺度无码无码专区| 2021国产麻豆剧果冻传媒入口| 久久久久人妻精品一区三寸蜜桃 | A一区二区三区乱码在线| 精品欧美h无遮挡在线看中文| 18禁国产精品久久久久久久| 特黄做受又大又粗又长大片| 色一情一乱一伦| 免费高清自慰一区二区三区| 伊人久久精品中文字幕无码| 国产精品久久久久久AV下载 | 亚洲熟妇综合久久久久久| 欧美日韩第一区| 国产精品无遮挡| 精品国产免费久久久久久婷婷| 蜜桃av网| 日韩av天堂| 日本一区二区在线播放| 太保市| 亚洲香蕉网久久综合影院小说| 亚洲另类无码一区二区三区| 国模国产精品嫩模大尺度视频| 人妻精品肉动漫h无码| 印度毛茸茸BBBBXXXX| 亚洲制服丝袜中文字幕在线| 中文字幕中文有码在线|